Which of the following can you conclude from your experiment? You have isolated a new species of mRNA using an oligo dT column. You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns. Below is a representation of the electron micrograph results.
A. The gene contains no introns B. The gene contains one intron C. The gene contains two introns. D. The gene contains three intron E. The results are inconclusive
The correct answer to this question is C; The gene contains two introns. Introns are sequences that are non-coding. These sequences are found within a gene. They are removed through the process of RNA splicing, which results in a final RNA of a gene.
The intron is a word that refers to the RNA transcripts as well as the DNA sequence. Most of the viruses and organisms contain these genes of introns. They are the opposite of the term exons, which are also parts of genes, but those are expressed, while introns are discarded. Biochemist Walter Gilbert introduced the term intron.