How does RNA polymerase performs a template directed synthesis?

RNA polymerase is a term used in molecular biology and can be abbreviated as RNAP. RNA polymerase refers to an enzyme that manufactures RNA from a DNA template. RNA polymerase uses the enzyme helicase to locally open the DNA that is double-stranded in order to use a strand of the exposed nucleotides as a template for the synthesis of RNA. This is a process described as transcription.

Hence, we can say RNA polymerase performs a template-directed sequence through the transcription process. Before RNA polymerase can start the DNA unwinding at the position, there must be an attachment of a transcription factor as well as the transcription mediator complex associated with it to a promoter.

A promoter can be explained as a DNA binding site. RNA polymerase also facilitates elongation and attachment, guides the positioning of the nucleotides. It, as well, has termination recognition capability, replacement capabilities, and intrinsic proofreading.

One strand of DNA, the template strand, is employed as a template for RNA synthesis. RNA polymerases transport the template strand and use base pairing complementarity with the DNA template to create an RNA copy. RNA polymerase is vital to life, and it is discovered in all living organisms and many viruses. Depending on the organism, an RNA polymerase can be a protein complex or consist of one subunit, each representing an independent ancestry.

DNA polymerase is a group of enzymes used to make copies of DNA templates, principally used in DNA replication mechanisms. These enzymes create new copies of DNA from present templates and operate by fixing the synthesized DNA to prevent changes.

The template produced synthesis method is an effective method to combine an array of aligned microfibers and nanofibers and nanowires with manageable length and diameter. The template synthesis method utilizes a nonporous membrane template containing pores of uniform diameter to produce nanofibers.