Can you explain how through genetic engineering of a bacterium, - ProProfs Discuss
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Can you explain how through genetic engineering of a bacterium, insulin is produced to treat diabetic patients?

This question is part of Biology IGCSE Quiz!
Asked by Bio, Last updated: Nov 12, 2020

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2 Answers

risinu911

risinu

risinu911
Risinu

Answered Feb 05, 2017

Isolate plasmids from bacteria the select the part of DNA that codes for the production of insuline. then using a restriction enzyme cut open thhe plasmid using same restriction enzyme cut part of the DNA then using DNA ligase jion the cut end of DNA ro plasmid to form recombinant plasmids. the incubate the recombinant plasmids with plasmid removed bacteria. some bacteria take up the recombinat plasmid and produce insuline.
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John Smith

John Smith

John Smith
John Smith

Answered Oct 19, 2016

1) A human insulin gene is indentified and isolated 2) This is done using restriction enzymes 3) The restriction enzyme cuts the plasmid 4) The plasmid is the vector 5) The human insulin gene and plasmid are joined together using ligase enzyme 6) This forms a recombinant plasmid 7) The plasmid is put back into the bacterium 8) The bacteria reproduces asexually 9) All offsprings will produce human insulin
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